FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37461163
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT3574550.0144.827061
ATAGGTC3353550.0144.687482
ATCGATT3275800.0144.656251
TACACGA2696450.0144.63892
TAAGTAG3771200.0144.531171
ATACACG2699200.0144.515211
ACTGTAT3111000.0144.502063
ACGATCG3295100.0144.490951
TCGATTC3281500.0144.484832
ACACGAT2699200.0144.463913
ATCGTTA2433200.0144.426214
TCGATAG3303100.0144.37515
TAGAGTC3772050.0144.342515
ATAGGTA3307950.0144.260678
TGTATCT3117650.0144.233185
CGATCAT3065450.0144.222781
TAGGTCG3354850.0144.222323
TCGATAA2828800.0144.197273
ATTCCTT3289900.0144.174455
TAACGCA2831700.0144.173667
Produced by FastQC (version 0.11.5)