FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39034459
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT3753350.0144.764941
ATCGATT3404850.0144.696561
ATACACG2812350.0144.619751
ACGATCG3452950.0144.616471
TAAGTAG3890550.0144.573011
TACACGA2813450.0144.536252
ACTGTAT3234900.0144.531723
ACACGAT2813450.0144.506243
TCGATTC3412500.0144.449462
ATAGGTC3497650.0144.339652
CGATCAT3215500.0144.33071
TGTATCT3238950.0144.314645
TAGAGTC3894800.0144.31155
TCGATCG2525750.0144.269441
TCGATAA2946450.0144.25123
ATCGTTA2525650.0144.239184
TCGCATC3505850.0144.233087
AATGGTC3757100.0144.158515
ACGATCT2822350.0144.150165
ATAGGTA3472250.0144.128318
Produced by FastQC (version 0.11.5)