FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34862008
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT3306600.0144.801181
ATCGATT3065300.0144.728261
TACACGA2509000.0144.702872
ATAGGTC3126200.0144.668982
ATACACG2511350.0144.589781
ACACGAT2510600.0144.562583
ACGATCG3089600.0144.549561
ACTGTAT2904700.0144.542163
TCGATAG3098650.0144.540085
TAAGTAG3488850.0144.509081
TCGATTC3072450.0144.477422
TCGATCG2250200.0144.367721
GTCGCAT3130750.0144.366786
ATCGTTA2250000.0144.344744
TAGAGTC3490250.0144.340455
TCGATAA2650900.0144.3353
TGTATCT2911050.0144.318595
ATAGGTA3105250.0144.285118
CGATCAT2826450.0144.261031
TAACGCA2651800.0144.259577
Produced by FastQC (version 0.11.5)