FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36167260
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT3458300.0144.795641
TACACGA2618750.0144.621572
ATACACG2620400.0144.616961
ACGATCG3156450.0144.57651
TAAGTAG3656500.0144.571371
ACACGAT2619000.0144.530643
ACTGTAT3003950.0144.470143
TCGATTC3102300.0144.397032
ATAGGTC3287600.0144.391562
TAGAGTC3660950.0144.314385
TGTATCT3009200.0144.27295
CGATCAT2960550.0144.227021
TCGTTAC2369300.0144.209235
ATAGGTA3175850.0144.170128
ATTCCTT3107500.0144.16645
CACGATC2626450.0144.15914
TAACGCA2720400.0144.14437
TAGGTCG3284300.0144.14143
ATCTCTT5656900.0144.11678
AACGCAT2722000.0144.089058
Produced by FastQC (version 0.11.5)