FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28182166
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT2449000.0144.592641
TCGATCG1699950.0144.562151
TAGGTCG2535050.0144.509723
TAACAAT2813800.0144.502661
TAAGTAG2928950.0144.496341
ACGATCG2457300.0144.437591
ATAGGTC2543550.0144.409412
TCGATTC2455550.0144.345322
ATCGTTA1700750.0144.3394
TCGATAG2470100.0144.328165
AAGTAGA2929500.0144.318952
TCGATAA2008600.0144.268633
ACTGTAT2384150.0144.250093
TACTGTA2385550.0144.205692
ATACACG2027300.0144.163441
GATCGTC2397850.0144.141278
ATGATCG2393700.0144.135336
ATCGTCT2400150.0144.123959
TCGTTAC1701050.0144.105515
TAGGTAA2472350.0144.08689
Produced by FastQC (version 0.11.5)