FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28366422
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAG2968250.0144.573171
TAACAAT2882450.0144.53611
ATCGATT2506900.0144.528371
ACGATCG2496850.0144.497541
TAGGTCG2583600.0144.486253
TCGATCG1792400.0144.480271
TCGATTC2510100.0144.459122
ATCGTTA1795400.0144.457284
AAGTAGA2971200.0144.376562
ATAGGTC2592750.0144.36342
TCGATAG2508100.0144.314835
ACTGTAT2394650.0144.294273
TACTGTA2396750.0144.262862
TCGTTAC1795750.0144.255815
ATACACG2053150.0144.232251
TCGATAA2028500.0144.23073
TGTATCT2397150.0144.142765
ATGATCG2288600.0144.14196
GATCGTC2290300.0144.130468
ATCGTCT2292750.0144.106589
Produced by FastQC (version 0.11.5)