FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24453818
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT2142250.0144.573011
TAAGTAG2573700.0144.544751
ATCGTTA1555700.0144.530214
ACGATCG2135500.0144.52421
TCGATCG1557050.0144.52411
TAGGTCG2198900.0144.503223
TCGATTC2144800.0144.498922
TAACAAT2456800.0144.471131
ATAGGTC2206250.0144.370652
ACTGTAT2046800.0144.309573
TCGTTAC1557050.0144.297845
AAGTAGA2576450.0144.276052
TACTGTA2048550.0144.261172
ATACACG1738850.0144.254471
TCGATAG2149700.0144.208245
TCGATAA1707350.0144.196293
ATGATCG2088350.0144.13436
ATCGTCT2091100.0144.124399
GATCGTC2089650.0144.119778
GTCGCAT2208700.0144.117146
Produced by FastQC (version 0.11.5)