FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_optHR_R20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_optHR_R20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32519242
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT3250800.0144.57381
ATCGATT2832900.0144.544661
TAAGTAG3377100.0144.533191
TCGATCG2057100.0144.518041
TAGGTCG2902550.0144.514393
ACGATCG2829850.0144.505751
TCGATTC2836800.0144.450262
ATAGGTC2912350.0144.413822
AAGTAGA3378750.0144.391052
ATCGTTA2058200.0144.390674
TCGATAG2845400.0144.308845
TACTGTA2706200.0144.280842
ACTGTAT2705350.0144.256763
ATCGTCT2778400.0144.255549
ATACACG2319800.0144.232821
ATGATCG2775500.0144.207346
TCGTTAC2058850.0144.1975
TCATGAT2780350.0144.17914
TCGATAA2331750.0144.150073
GATCGTC2779000.0144.145698
Produced by FastQC (version 0.11.5)