FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75356764
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15306042.0311434816919687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA952300.018.80163294-95
TATCATT1401650.018.08941794-95
GTATCAT1664900.017.23194794-95
ATGCCGT77050.016.7062854
CGTATCA1961550.016.44326294-95
TAGACGG54100.015.5402965
CCGTATC2295750.015.27234294-95
ATCATTA1487000.015.27190994-95
GTACCGT71550.014.9367516
AGTACCG92850.014.7330915
GTCTATG137900.014.3482191
GCCGTAT2809550.013.9899794-95
TACCGTG84250.013.8691057
CACTGAG153200.013.7039827
GTCTTAG107800.013.3688121
CGCCGTA3314000.013.13034194-95
TATACTG162500.013.03661255
GTATAGG152300.012.9915921
TACACCG96100.012.9497445
GTCCTAA129000.012.46223451
Produced by FastQC (version 0.11.5)