FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_POK60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_POK60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82754666
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG102750.028.3381085
GTCTAGG244800.022.2802371
TATACCG281400.021.5893065
CCTAGAC242300.019.4076163
GTACCGT154500.018.938456
GTCTATG303500.018.7067581
ACTGTCC153450.018.6346748
TAGAGCG243700.018.6335075
ATAGACG133150.018.586124
GACGGTC138650.018.4997627
GTATCAT2266550.018.46730494-95
AGTACCG225850.018.4447065
CTAGACA301300.018.0824184
CGTATCA2695850.018.00733894-95
ACTGAGC344650.017.8064148
TATCATT1960750.017.74757694-95
CACTGAG359150.017.6958927
ATCATTA1608900.017.64756894-95
GTGTATG366200.017.5926271
GGACTGC198150.017.4273996
Produced by FastQC (version 0.11.5)