FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_POK60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_POK60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88233825
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4138550.024.67083594-95
CATTAAA2926150.023.76344794-95
ATCATTA5555250.023.16071194-95
TAGACGG89100.022.070685
TATCATT7068600.021.02951294-95
TATACCG214850.020.6713755
GTACCGT135750.019.4548596
GTCTAGG200550.019.0945361
GTATCAT8685800.018.70981894-95
AGTACCG184600.017.5487355
TAGAGCG210600.017.3444735
GTCTATG249450.016.837031
ACTGTCC137000.016.6423178
TACCGTG171250.016.2817337
ATTAAAA2359500.016.23700594-95
CGTATCA10466600.016.20588594-95
CTAGACA222750.016.1425174
CCTAGAC214100.016.1067873
GTATAAG330900.015.7653041
CACTGAG281250.015.5715387
Produced by FastQC (version 0.11.5)