FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_POK5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_POK5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66554589
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA1705500.018.50705794-95
CATTAAA1376800.017.93844494-95
ATCATTA2056500.016.63252494-95
CCGTATC4100700.016.30942794-95
CGTATCA3363100.016.23540994-95
GTCTAGG170250.015.7672731
GTATCAT2834750.015.66885894-95
GCCGTAT5143200.015.57521794-95
TATACCG193550.015.3139345
TATCATT2425850.015.26322394-95
CGCCGTA6190950.014.60536894-95
GTACCGT147750.014.5635116
TAGAGCG179700.014.2209825
GTATAGG281800.013.8250941
CCTAGAC170150.013.734833
ACTGTCC147050.013.566878
AGTACCG178300.013.5334255
GTCTATG232150.013.4254971
ACTGAGC282050.012.9339298
TATACTG285200.012.8243865
Produced by FastQC (version 0.11.5)