FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_POK180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_POK180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80531034
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG99600.026.7068025
GTCTAGG231550.021.4421221
TATACCG257300.020.6208525
CCTAGAC228200.019.3786953
TAGAGCG227500.018.853835
GTATCAT2225300.017.70070894-95
ATAGACG128800.017.628094
GTCTATG280300.017.4755881
GTACCGT147350.017.4719896
CGTATCA2629900.017.47001394-95
ACTGTCC151600.017.2641688
GACGGTC138500.017.1822957
ATCATTA1608700.017.15368594-95
CCGTATC3144750.017.02357794-95
AGTACCG207600.017.0231065
CACTGAG338400.016.9843227
TATCATT1958800.016.94679694-95
TATACTG355000.016.912665
ACTGAGC320550.016.8780068
CTAGACA282050.016.7062424
Produced by FastQC (version 0.11.5)