FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_POK15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_POK15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73582977
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG82650.025.6321755
TATCATT2683050.022.03143394-95
ATCATTA2026500.021.6662694-95
GTATCAT3336050.021.48718694-95
GTCTAGG202700.020.2044261
GTACCGT133750.019.8878466
TCATTAA1553300.019.50550894-95
CTAGACA221500.019.0214394
CGTATCA4125250.019.01038494-95
CCTAGAC205900.018.8014073
TATACCG206550.018.4204755
ACTGTCC133250.018.2870488
AGTACCG187300.018.0312275
GTCTATG238400.017.5574991
TAGAGCG210650.017.1374265
GTATAAG312400.017.0485531
CATTAAA1143550.016.97424394-95
CACTGAG289650.016.7926777
CCGTATC4952900.016.73276594-95
ACTGAGC286750.016.6146438
Produced by FastQC (version 0.11.5)