FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_POK15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_POK15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84257784
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3385200.022.62464594-95
CATTAAA2467650.021.96607694-95
TAGACGG89950.021.4924325
ATCATTA4361900.020.92355294-95
TATACCG229300.020.7151575
GTACCGT135750.020.5045436
GTCTAGG210650.019.8029211
AGTACCG186450.019.6928885
TATCATT5369100.018.70237594-95
TAGAGCG212200.018.4448095
GTCTATG254400.017.2190821
GTATCAT6408550.017.0423494-95
CCTAGAC214350.016.8858033
TACCGTG175800.016.8329987
ACTGTCC138200.016.8071128
GTGTATG307950.016.4002041
CACTGAG292250.016.2206527
GTATAAG338150.016.0595361
ACTGAGC291350.015.7979618
ATTAAAA2057550.015.52743694-95
Produced by FastQC (version 0.11.5)