FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66473772
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG85200.034.4540335
GACGGTC98850.027.0054137
GTCTAGG211500.023.0030421
ATAGACG126000.020.9978984
CCTAGAC216750.020.9940533
TATACCG256150.020.4908075
GTACCGT121050.020.2477176
GCCCTAG119150.019.2596681
CTAGACA263150.019.1334694
GTCTATG278500.018.9532891
ACTGTCC126650.018.9023828
AGACGGT147300.018.8644586
GGACTGC158250.018.3995826
AGCGCGC122000.018.3380138
AGTACCG179200.017.9449335
TAGAGCG221000.017.6458285
GTGTATG317300.017.1297761
GTATCAT1367750.017.06216694-95
ACGGTCC166900.017.0190778
CACTGAG321600.016.8080527
Produced by FastQC (version 0.11.5)