FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175657463
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG162250.027.6070545
TCATTAA6199250.024.17662894-95
ATCATTA8257700.023.33501294-95
GTACCGT245600.023.3244846
CATTAAA4286300.022.66678494-95
TATACCG488600.021.7084275
TATCATT10403050.020.94093194-95
TACCGTG318450.020.3901487
AGTACCG358900.020.1699435
GTCTAGG458050.019.7701641
ACTGTCC244300.019.6377168
GGACTGC325900.019.2098586
GTATCAT12539000.018.55491494-95
TAGAGCG425200.018.3542825
GTCTATG541100.018.289931
CACTGAG612400.018.11117
ACTGAGC558200.017.861418
AAGACCG370850.017.7140145
GACGGTC212600.017.6504977
CTAGACA477500.017.3984094
Produced by FastQC (version 0.11.5)