FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58602060
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2380650.017.14025594-95
TATACCG186150.016.8922625
GCCGTAT3138650.016.60872394-95
CGTATCA1936300.016.35874294-95
GTCTAGG182350.015.7617341
CGCCGTA3786700.015.68617994-95
TAGAGCG182750.015.6730235
TCATTAA1087150.015.3185294-95
TAGACGG81550.015.0858125
CCTAGAC168750.014.8621463
GTCTATG224000.014.8458281
GTATCAT1674650.014.79617794-95
CATTAAA907400.014.79339194-95
ATCATTA1281300.014.06318494-95
GTACCGT133500.013.8763816
ACTGTCC147850.013.4291298
TATCATT1511850.013.34972794-95
ACTGAGC275800.013.0547288
CACTGAG283600.013.0474047
CTAGACA250950.013.041424
Produced by FastQC (version 0.11.5)