FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72744700
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2080650.020.90037794-95
ATCATTA2649550.019.96868194-95
CATTAAA1564050.019.4504494-95
TATCATT3302150.018.26269794-95
GTATCAT3936800.017.79927694-95
CGTATCA4722650.016.94511694-95
TATACCG181450.016.6491815
CCGTATC5681400.015.74266394-95
TAGACGG95200.015.2179315
GTCTAGG202000.015.2172151
GCCGTAT6888150.014.44384994-95
GTACCGT133650.013.9674236
AGTACCG160850.013.8498325
GTATAAG274950.013.3396931
CGCCGTA8101800.013.28564894-95
TACACCG168200.013.2446225
TATACTG321250.013.2334375
ACTGAGC246150.013.006278
GTATAGG252500.012.9452171
CCTAGAC200400.012.9415113
Produced by FastQC (version 0.11.5)