FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105261654
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1384700.13154837943169695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG136100.022.685485
TATACCG286350.022.4934065
GTCTAGG298100.022.058261
GTCTATG346100.021.2503721
TAGAGCG313700.021.1228475
ATCATTA2249350.021.00318594-95
TCATTAA1670950.020.8412294-95
TATCATT2835000.020.1418594-95
CTAGACA325250.019.9638334
GTATCAT3484000.019.55081294-95
GTACCGT204050.018.8323556
AGTACCG264550.018.7455
CGTATCA4287000.018.6360394-95
CCTAGAC303150.018.551723
CATTAAA1215750.018.13068894-95
CACTGAG518800.017.9543787
ACTGAGC478650.017.9222458
CCGTATC5302800.017.72203494-95
GTGTATG403100.017.5265051
TACACCG254850.017.2964135
Produced by FastQC (version 0.11.5)