FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108586553
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG121050.031.5096245
TATACCG340900.023.4224975
GTCTAGG314450.023.282861
GTACCGT173100.022.0348936
TAGAGCG293700.021.1703575
GTCTATG374800.021.0423281
GACGGTC156800.020.9023697
AGTACCG252700.020.6202455
GGACTGC237500.020.2999486
ATCATTA2571850.019.90705394-95
CCTAGAC316800.019.6565843
ATAGACG164900.019.443554
CTAGACA356600.019.4341894
ACTGTCC177450.019.4068248
CACTGAG428900.019.4030727
TATCATT3189000.019.19143194-95
TCATTAA1999050.019.18370494-95
ACTGAGC387000.019.098148
GCCCTAG198000.019.0759961
GTATCAT3748050.018.9902794-95
Produced by FastQC (version 0.11.5)