FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83188243
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG104650.031.1370535
GACGGTC130150.021.8247687
GTCTAGG266650.021.025021
TATACCG309750.020.9474895
GTCTATG333650.020.4341581
CCTAGAC265500.020.0553653
TAGAGCG262800.019.863925
GTACCGT157750.019.812956
GCCCTAG172600.019.3237921
ATAGACG148200.019.0063674
CTAGACA332750.018.6716354
AGTACCG231500.018.343375
AGCGCGC178750.018.2824768
GGACTGC202900.018.2133646
ACTGTCC160200.018.2053288
CACTGAG400050.017.7508667
CCGTATC2332700.017.70535594-95
CGTATCA1986000.017.52430794-95
ACTGAGC359850.017.5162858
GTATCAT1729650.017.2792394-95
Produced by FastQC (version 0.11.5)