FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_CUI15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_CUI15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131669559
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG137550.023.827625
TCATTAA4463400.023.17481694-95
CATTAAA3191400.022.0919794-95
ATCATTA5825750.021.59363694-95
TATACCG371300.019.9824285
GTCTAGG354650.019.9742131
GTACCGT202200.019.544946
TATCATT7186250.019.10577694-95
AGTACCG282500.018.4282575
TAGAGCG307400.017.7390445
TACCGTG255050.017.7111687
ACTGTCC213050.017.6131738
GTATCAT8541450.017.42994594-95
GTCTATG407400.017.2013681
TACACCG263950.017.0599945
CACTGAG451650.016.9322977
ACTGAGC416750.016.743198
CCTAGAC359000.016.4197943
CTAGACA399600.016.296874
GGACTGC264250.015.9800816
Produced by FastQC (version 0.11.5)