FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101718024
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1662440.16343612809466293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG141600.022.8106635
ATCATTA2189900.021.24906794-95
TATCATT2804850.020.61913394-95
TCATTAA1653900.020.48882994-95
TATACCG280700.020.3063145
GTATCAT3442700.020.05368294-95
GTCTAGG265050.019.9508441
GTACCGT198700.019.2915866
CGTATCA4211500.018.5178294-95
CCTAGAC283400.018.3026073
GTCTATG325050.018.0806241
CTAGACA318350.017.979374
TAGAGCG298600.017.8482385
ATAGACG138450.017.5658534
ACTGTCC204150.017.5434198
CATTAAA1200250.017.41500394-95
GTGTATG373550.017.081311
CCGTATC5111650.016.97737994-95
AGTACCG278150.016.8379865
TACACCG243750.016.7394355
Produced by FastQC (version 0.11.5)