FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131382185
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1878850.1430064509887699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4518150.023.92166394-95
CATTAAA3231900.022.80206394-95
ATCATTA5952500.022.59694794-95
TATCATT7509150.020.32771394-95
GTATCAT9099300.018.23438394-95
TATACCG310250.017.1780745
GTCTAGG314350.017.0788211
TAGACGG165850.016.3249865
CGTATCA10860300.015.88387294-95
GTACCGT223100.015.286866
ATTAAAA2737400.014.83097594-95
ACTGTCC237400.014.4260678
GTATAAG454950.014.338371
CCGTATC12765100.014.12971894-95
GTCTATG374900.014.0542951
CCTAGAC324850.014.0371293
ACTGAGC491100.013.9665998
CTAGACA373950.013.8835344
TATACTG475550.013.7740455
GTGTATG430000.013.7671031
Produced by FastQC (version 0.11.5)