FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74745205
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2279600.3049827744803162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2042950.016.86953294-95
GCCGTAT2701050.016.61589494-95
CGTATCA1634250.015.53395594-95
CGCCGTA3368200.015.35971994-95
CATTAAA902800.013.59547694-95
TCATTAA1024550.013.56082694-95
GTATCAT1441550.012.9413694-95
GTCTAGG209250.012.4424821
TATACCG203500.011.9041735
ATCATTA1151850.011.38374894-95
TATACTG266400.011.3757475
TAGAGCG234400.011.0846815
ACTGTCC205500.010.9562018
GTATAGG278950.010.9515741
TCGCCGT4488950.010.92596394-95
TGGTCGC4800850.010.87309690-91
GTGTATG330400.010.7992071
CCTAGAC197300.010.7614263
TATCATT1302200.010.74604194-95
GTATAGA251000.010.5053661
Produced by FastQC (version 0.11.5)