FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50843503
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1563860.3075830554004117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1752500.015.31789294-95
CATTAAA717450.015.15470494-95
CCGTATC1375100.014.47348394-95
CGCCGTA2209900.014.46665194-95
TCATTAA782600.013.97807694-95
CGTATCA1123200.013.14368794-95
ATCATTA853650.012.48359192-93
GTCTAGG139100.011.4764491
TATCATT940150.011.28449492-93
TATACTG166700.010.913315
GTATAGG168250.010.5894061
GTATCAT1017450.010.57655694-95
GTATTGG250900.010.5664461
ATTAAAA829650.010.53741894-95
TGGTCGC3081050.010.49499490-91
TCGCCGT3069950.010.15541194-95
TATACCG130850.010.05545
ACTGTCC129650.010.0385588
CCTAGAC140250.09.9232563
CTATACT192050.09.7695844
Produced by FastQC (version 0.11.5)