FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87179749
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG110550.024.1044045
GTCTAGG256550.020.6857051
TATACCG272250.020.4306095
CCTAGAC250700.018.7004973
GTACCGT170400.018.3420816
TAGAGCG252900.018.312525
GTCTATG308900.018.1797871
GTATCAT2546250.017.79395194-95
AGTACCG236500.017.6542825
CGTATCA3025850.017.63441394-95
ATCATTA1787350.017.51334694-95
TATCATT2177600.017.3489694-95
CCGTATC3680450.017.34375694-95
CACTGAG385450.017.289487
CTAGACA322950.017.2084944
ACTGAGC367350.017.0422788
TCATTAA1433300.016.89988794-95
ATAGACG131200.016.4366874
ACTGTCC171600.016.1377748
GCCGTAT4819500.015.84120794-95
Produced by FastQC (version 0.11.5)