FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135266885
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA5395250.025.23243594-95
CATTAAA3785850.024.37582294-95
ATCATTA7292050.023.31671194-95
TATCATT9239250.020.83593694-95
TATACCG316350.019.579535
TAGACGG154300.019.116935
GTCTAGG309700.018.7772121
GTATCAT11345200.018.41983694-95
GTACCGT215500.017.699496
TAGAGCG328000.017.6676375
ATTAAAA2868700.017.57803794-95
ACTGTCC223850.016.6360998
AGTACCG301100.016.2171615
CGTATCA13624300.016.03877694-95
GTCTATG373550.015.7075741
CCTAGAC323900.015.6181333
GTATAAG448200.015.5930731
GTCTTAG301500.015.4271711
GTGTATG425100.015.2668881
TACACCG268300.015.1369345
Produced by FastQC (version 0.11.5)