FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100914883
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2298890.227804852134645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA2171950.022.28968494-95
TATCATT2837150.021.31279894-95
TCATTAA1665150.021.00369894-95
GTATCAT3493350.020.60872794-95
CGTATCA4326550.018.83842594-95
GTCTAGG260700.017.7502731
CATTAAA1231400.017.68041494-95
CCGTATC5294050.016.98511194-95
TATACCG221500.016.8126035
TAGACGG145100.016.662615
CCTAGAC258700.015.9556093
TACACCG253150.015.2547555
GCCGTAT6594800.015.16411394-95
CTAGACA282750.015.102534
GTATAAG313500.015.0183841
GTGTATG320950.014.8770141
GTATTGG452000.014.8732231
GTACCGT191250.014.7529086
ACTGTCC196500.014.7455148
TATACTG334250.014.6798485
Produced by FastQC (version 0.11.5)