FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n02_AZU15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n02_AZU15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136904437
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4450950.022.77647894-95
ATCATTA5760350.021.81941894-95
CATTAAA3212950.021.26299594-95
TAGACGG145250.020.7658925
TATCATT7178650.019.6834794-95
TATACCG361900.019.0839735
GTCTAGG343750.018.921631
GTATCAT8598650.018.00617294-95
GTACCGT209700.017.1017886
CGTATCA10286950.016.064394-95
AGTACCG298800.015.7697225
GGACTGC289900.015.6646
GTATAAG576250.015.3273221
AAGACCG304450.015.1806335
TACCGTG282050.015.1231837
CCTAGAC354350.015.1071013
ACTGTCC221000.014.9807468
CCGTATC12139550.014.71988694-95
CTAGACA422000.014.7002164
CACTGAG452200.014.6848527
Produced by FastQC (version 0.11.5)