FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75356764
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG127550.020.5436381
GTACCGT63900.017.783046
TAGACGG51950.017.4806295
ACTGTCC70600.017.010428
AGTACCG89600.016.980535
CACTGAG145900.015.963457
GTGTATG129550.015.894861
TACCGTG82850.014.8591257
TACACCG96400.014.746995
GTCTTAG87800.014.4076561
GTCGGTC162300.013.833818
GACAGTG81850.013.8211547
GCCCTAG88900.013.8014321
ACCGTGA114800.013.7689728
TAGAGGT107900.013.7492694
GTGTAGG88700.013.6180931
TAAGGTG116950.013.5786155
TATACTG157150.013.372645
ACAGTGC111700.013.3436818
ACTGTGC87600.013.3300018
Produced by FastQC (version 0.11.5)