FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_POK60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_POK60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82754666
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG106150.031.9551725
GTCTAGG244800.024.7267421
TATACCG281250.023.1583615
GACGGTC137200.022.1564737
GTCTATG308700.021.2860181
ATAGACG137200.021.2277364
GTACCGT163950.021.095156
CCTAGAC251600.021.0930733
CTAGACA301750.020.1224524
TAGAGCG250300.019.872335
GGACTGC207400.019.7909976
ACTGAGC349000.019.5703938
ACTGTCC154500.019.4599328
TATACTG372500.019.1943385
CTTCTGC1515900.019.12040194-95
CACTGAG381800.019.046457
GTATAAG410950.019.0304781
AGTACCG232150.018.9088975
TACACCG185700.018.3429935
GTGTATG362550.018.1899031
Produced by FastQC (version 0.11.5)