FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_POK60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_POK60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88233825
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2836500.024.99245894-95
CTTCTGC4237500.023.4646394-95
TCTGCTT2189050.023.33600694-95
TAGACGG90500.022.9410785
TATACCG216950.021.526575
TCTTCTG5533700.020.98723494-95
GTCTAGG207050.020.1719231
GTACCGT130900.019.744236
CTGCTTG1331300.019.35779494-95
GTCTTCT6755100.018.92581694-95
AGTACCG185050.018.870325
ACTGTCC134950.018.407498
TCTGCGT310200.017.6707994-95
CCTAGAC212450.017.4446373
GTATAAG331450.017.4034081
GTCTATG239750.017.3809951
ACTGAGC289550.017.355138
TTCTGCG362750.017.27801994-95
CACTGAG293150.017.2719447
TAGAGCG209150.017.1956655
Produced by FastQC (version 0.11.5)