FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_POK5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_POK5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66554589
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT1746050.018.62392494-95
TCTGCTT1409850.017.81765794-95
CTTCTGC2140800.016.60759494-95
TATACCG192000.016.1330815
CCGTCTT4428350.015.85933894-95
CTGCTTG1035250.015.81794494-95
GTCTAGG166450.015.6712821
GCCGTCT5397600.015.61279394-95
TAGACGG92550.015.6051565
GTCTTCT3076100.015.30951194-95
CGTCTTC3777400.015.23363494-95
TCTTCTG2620950.015.16087194-95
TAGAGCG181800.015.0260535
TGCTTGA793500.014.94130994-95
ACTGTCC149000.014.69543658
TGCCGTC6452600.014.46979794-95
GTACCGT151450.014.3984036
GCTTGAA629300.014.16386694-95
GTATAGG283200.014.1265071
GTCTATG224950.013.98261
Produced by FastQC (version 0.11.5)