FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_POK180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_POK180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80531034
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG103800.028.835095
GTCTAGG226300.023.6628681
TATACCG266050.021.4287155
CCTAGAC240300.020.958943
TAGAGCG245100.020.5854025
GTACCGT149850.020.2274936
ACTGAGC332750.019.7268058
CACTGAG354500.019.280557
AGTACCG213000.019.2267465
GTCTATG280450.019.1448081
ATAGACG129800.019.1079044
TATACTG354500.018.7758675
ACTGTCC145500.018.7703178
GACGGTC143100.018.6539827
GGACTGC194400.018.5980116
CTTCTGC1492800.018.43607594-95
GTGTATG339550.018.261431
GTATAGG395800.017.8990461
GTATAAG384450.017.4016651
GTCTTCT2302650.017.29786994-95
Produced by FastQC (version 0.11.5)