FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_POK15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_POK15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73582977
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG79300.026.1205545
CTTCTGC1997300.022.38501794-95
GTCTAGG196950.021.9533651
TCTTCTG2813600.021.75546394-95
TTCTGCT1441700.021.08796794-95
GTCTTCT3507900.020.8672594-95
GTACCGT134900.020.3204176
TATACCG210350.020.281635
ACTGTCC132150.019.8398328
CCTAGAC211450.019.7508453
CTAGACA222950.019.1156674
GACAGTG131250.018.7818957
TAGAGCG203750.018.6302325
AGTACCG184700.018.4940095
CGTCTTC4352200.018.42841194-95
GTCTATG238400.018.1164591
GTATAAG316450.017.9723451
ACTGAGC286250.017.8207048
GTGTATG257700.017.3496571
TCTGCTT1116950.017.180894-95
Produced by FastQC (version 0.11.5)