FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_POK15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_POK15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84257784
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG93200.025.487425
TTCTGCT3317000.023.23159494-95
TCTGCTT2447900.021.98612494-95
CTTCTGC4361100.021.12888794-95
GTCTAGG204900.020.9628141
TATACCG227250.020.4668315
GTACCGT140250.020.4261116
CTGCTTG1636350.019.38344694-95
AGTACCG190150.018.5636885
TCTTCTG5581450.018.46911294-95
ACTGTCC144050.018.4317028
CCTAGAC223400.018.2267253
TAGAGCG210350.018.2038945
GTATAAG344500.017.4057671
GTCTATG256900.017.3854851
TGCTTGA1182350.017.37132594-95
ACTGAGC292850.017.3217958
CACTGAG299700.017.1797227
TACCGTG180400.017.1666157
GTGTATG298650.016.8005891
Produced by FastQC (version 0.11.5)