FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66473772
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG85100.034.5012635
GACGGTC100700.027.4043527
GTCTAGG215550.026.451951
GTACCGT130200.022.5138766
CCTAGAC221150.021.914253
ATAGACG124750.021.6713054
ACTGTCC131250.021.3510078
AGTACCG193250.020.872015
GTCTATG266950.020.8603761
GGACTGC158800.020.5832566
TATACCG258600.020.465995
CTAGACA269550.020.0945784
GCCCTAG124500.019.9979921
AGACGGT146900.019.0812286
CACTGAG336600.018.767677
GTGTATG310500.018.561951
TACCGTG169250.018.5500747
TAGAGCG225500.018.4769025
ACTGAGC307150.018.4482698
AGCGCGC124750.018.3515248
Produced by FastQC (version 0.11.5)