FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175657463
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG162900.028.2316235
TTCTGCT6012400.025.68792394-95
CTTCTGC8302050.023.99127694-95
GTACCGT248900.023.802536
TCTGCTT4238800.023.39586494-95
TATACCG486250.022.7166845
GTCTAGG454900.022.5198291
AGTACCG365800.020.8715065
TCTTCTG11044650.020.76559694-95
GGACTGC334850.020.6014546
ACTGTCC244800.020.5082368
TACCGTG327400.020.4556247
TAGAGCG431100.019.6827165
CTGCTTG2629450.019.3390594-95
AAGACCG377050.018.7997235
GTCTATG531900.018.661251
ACTGAGC562500.018.5005478
CACTGAG619200.018.4713157
GTCTTCT13314500.018.27701894-95
GTATAAG788150.018.0137441
Produced by FastQC (version 0.11.5)