FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58602060
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG186850.016.499845
TAGACGG85300.016.0944795
GCCGTCT786350.015.83243794-95
CCGTCTT826600.015.82003394-95
GTACCGT137600.015.53537756
CCTAGAC181450.015.1594733
GTCTAGG179050.014.9915171
CACTGAG282400.014.5490147
ACTGAGC270800.014.400318
AGTACCG166350.014.278265
GTCTATG221450.014.2450461
TGCCGTC1218200.014.2380194-95
TTCCGTC718100.013.97691194-95
TAGAGCG176400.013.9764495
TATACTG274950.013.4071385
TCCGTCT534100.013.33139794-95
ACTGTCC153050.013.189648
GTGTATG284400.012.4617981
CGTCTTC749800.012.44840594-95
GTATAAG259050.012.2874751
Produced by FastQC (version 0.11.5)