FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72744700
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2065500.021.2008194-95
CTTCTGC2675550.020.04532894-95
TCTGCTT1571950.019.38588394-95
TCTTCTG3419000.018.2497994-95
GTCTTCT4126400.017.38659394-95
TATACCG184250.016.7860985
TAGACGG96750.016.5974735
CTGCTTG1108700.016.31675394-95
CGTCTTC5047600.016.23297194-95
CCGTCTT5908000.015.48293594-95
GTCTAGG200650.015.0135591
TAGAGCG167150.014.4673065
GCCGTCT7068050.014.407594-95
TGCTTGA853300.014.28396594-95
GTACCGT140900.013.9593636
AGTACCG164000.013.9050865
ACTGAGC252650.013.79879958
GTATAGG252850.013.39862251
ACTGTCC154100.013.2226738
GCTTGAA666550.013.16218594-95
Produced by FastQC (version 0.11.5)