FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105261654
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG142600.025.2539165
GTCTAGG309750.022.487871
TATACCG281450.022.2312345
GTCTATG348500.021.7734761
TTCTGCT1724550.020.61894894-95
TAGAGCG310750.020.5784635
GTACCGT213900.020.1675856
CTTCTGC2419450.020.11252494-95
AGTACCG271550.019.9799945
ACTGAGC482400.019.9184328
TCTTCTG3040150.019.7856994-95
CACTGAG512200.019.0937047
CCTAGAC301250.019.0526143
GTCTTCT3751800.019.01235694-95
ATAGACG146950.018.7531034
ACTGTCC227750.018.2688968
TACACCG252000.018.1930625
GTCTAGA272450.018.1372811
GTATAAG370300.018.0408611
GTGTATG415500.017.5077291
Produced by FastQC (version 0.11.5)