FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108586553
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG117300.031.6313325
GTCTAGG310750.024.5553591
TATACCG337600.022.571815
GTACCGT179850.022.5585776
TAGAGCG292000.021.9479315
AGTACCG258050.021.6689135
GACGGTC155450.021.0830427
CTTCTGC2317350.020.99363994-95
ACTGAGC403550.020.7737568
ACTGTCC183400.020.7702988
GTCTATG374400.020.6726971
TTCTGCT1726450.020.43815694-95
CCTAGAC324550.020.4364433
GGACTGC241950.020.3618586
CACTGAG443850.020.1720497
GCCCTAG194600.020.0208661
TACCGTG228100.019.9487237
TACACCG222400.019.7379345
CTAGACA357050.019.5077174
TCTTCTG3045600.019.46340894-95
Produced by FastQC (version 0.11.5)