FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83188243
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG103200.035.54035
GACGGTC132400.024.5740497
GTCTAGG264300.024.4856261
ATAGACG148450.022.2126484
GTACCGT162900.021.844586
CCTAGAC263750.021.509633
TATACCG311000.020.8676625
AGTACCG232600.020.6502325
GTCTATG315400.020.5185531
TAGAGCG266200.020.1140425
CTAGACA344250.019.7923014
GGACTGC201800.019.5877786
ACTGTCC157150.019.3131338
TACACCG192750.019.0039335
CACTGAG401900.018.9447827
ACTGAGC363900.018.7560338
GTGTATG399150.018.4036771
GCCCTAG164050.018.3340641
AGACGGT192000.018.0636126
GTATAAG444100.017.760641
Produced by FastQC (version 0.11.5)