FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_CUI15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_CUI15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131669559
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG141500.024.4764085
TTCTGCT4373450.024.09611394-95
TCTGCTT3171250.022.34158794-95
GTACCGT206600.022.167836
CTTCTGC5818300.022.15102894-95
TATACCG372150.021.7279195
GTCTAGG352600.020.7119161
AGTACCG286150.019.7407995
CTGCTTG2051050.019.25190594-95
TCTTCTG7505300.019.03050494-95
TACCGTG259700.018.7102037
TAGAGCG312050.018.3915945
TACACCG259400.017.325985
ACTGAGC421250.017.1947638
GTCTTCT8964200.017.15339994-95
CACTGAG462500.017.11987
GTCTATG403650.017.056591
ACTGTCC218400.016.9849668
CCTAGAC367100.016.9811783
GGACTGC276200.016.8053426
Produced by FastQC (version 0.11.5)