FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101718024
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG137600.023.5818465
GTCTAGG281800.022.7623331
TATACCG290100.021.3061545
TTCTGCT1641350.020.90013394-95
CTTCTGC2285650.020.7319294-95
GTACCGT206100.020.1699856
GTCTATG322200.020.1589071
CCTAGAC280150.020.0974853
TCTTCTG2976100.020.0782594-95
CTAGACA330250.020.041134
ACTGTCC203450.019.820648
GTCTTCT3677000.019.32614594-95
GTGTATG380050.018.7281571
TAGAGCG307050.018.6291455
TACACCG249900.018.4408535
ACTGAGC462600.018.2863068
GTATAAG381250.018.2454761
CACTGAG474400.017.9819537
TATACTG372500.017.9705035
GACAGTG263000.017.843357
Produced by FastQC (version 0.11.5)