FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131382185
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT4424050.024.2790594-95
TCTGCTT3208850.022.54837694-95
CTTCTGC6057300.022.33337894-95
TCTTCTG7857200.019.86158894-95
CTGCTTG2118150.018.78000594-95
TATACCG321450.018.4595785
GTCTAGG321150.018.0797081
GTCTTCT9474850.017.74221694-95
TAGACGG161850.016.966335
GTACCGT237950.016.6115596
GTATAAG469550.016.008581
ACTGTCC239150.015.868788
AGTACCG318950.015.759265
CCTAGAC328850.015.7487613
GTCTATG374450.015.6457861
TGCTTGA1455450.015.57224394-95
CGTCTTC11546400.015.22695194-95
TAGAGCG340050.014.8233175
TATACTG480150.014.69345
GGACCGT329450.014.5936456
Produced by FastQC (version 0.11.5)