FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74745205
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2876650.016.12667894-95
CCGTCTT2247700.015.7533694-95
TGCCGTC3509150.014.9498694-95
GTCTAGG200100.013.7482851
TATACCG214250.013.3932995
ATGCCGT3747900.013.12936894-95
TATACTG273450.012.8044525
TATGCCG4150000.012.58923292-93
CCTAGAC201600.012.5144673
TTCTGCT1124200.012.46228994-95
CGTCTTC2131850.012.41167994-95
TCTGCTT1012050.012.01989394-95
GTCTTCT1627250.011.95343394-95
TGCTTGA650400.011.91880394-95
GCTTGAA526600.011.74870294-95
ACTGTCC206250.011.6986348
TAGAGCG227400.011.6577635
CTATACT302150.011.2589734
CGTATGC4628450.011.25495290-91
GTCTATG256300.011.1414821
Produced by FastQC (version 0.11.5)