FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50843503
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA490000.014.39035894-95
GCCGTCT1918150.014.36878594-95
GCTTGAA379950.014.16416394-95
TCTGCTT792550.013.82038394-95
TGCCGTC2355700.013.72836894-95
CCGTCTT1509450.013.56584294-95
CTGCTTG659550.012.8371794-95
ATGCCGT2445800.012.69341894-95
TTCTGCT870800.012.54575694-95
TATACTG168050.012.4109585
TATGCCG2688400.011.98880492-93
GTCTAGG140750.011.9167471
GTATTGG261350.011.7265061
TATACCG132400.011.3749785
CCTAGAC136900.011.3492393
CGTATGC2906100.011.0612290-91
GTATAGG164650.011.0527021
GTATGCC2822750.011.01854292-93
CTTCTGC995450.010.99387892-93
GTCTTAG103400.010.9367391
Produced by FastQC (version 0.11.5)